Gene Cloning And Manipulation

Author : Christopher Howe
Publisher : Cambridge University Press
ISBN 13 : 1139463586
Total Pages : 250 pages
Book Rating : 4.84/5 ( download)

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Download or read book Gene Cloning and Manipulation written by Christopher Howe and published by Cambridge University Press. This book was released on 2007-07-12 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

Author : R. W. Old
Publisher : Univ of California Press
ISBN 13 : 9780520046269
Total Pages : 228 pages
Book Rating : 4.69/5 ( download)

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Download or read book Principles of Gene Manipulation written by R. W. Old and published by Univ of California Press. This book was released on 1981 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : David M. Glover
Publisher : Springer
ISBN 13 : 1489932461
Total Pages : 231 pages
Book Rating : 4.64/5 ( download)

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Download or read book Gene Cloning written by David M. Glover and published by Springer. This book was released on 2013-12-19 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book was originallyconceived in the form ofa second edition ofa volume published in 1980 in Chapman and Hall's 'OutllneStudies in Biology' series and entitled Genetic Engineering - Cloning DNA. It very rapidly became apparent that with the impact ofrecombinant DNA techniques being feIt in so many areas ofblology, it was going to be difficultifnotimpossible to keepthe bookwithin the space confines of these little monographs. The stays were therefore loosened and the book expanded comfortably to its present size. I hope that this extra space has allowed me to clarify sections ofthe text that were 'heavy going' in the earlierversion. Theextraspace has certainlyallowed me to cover topics that were not mentioned at all in the earlier book. These are primarily to be found in Chapters 7 and 8, which cover the rapid advances that have been recently made in the use ofplantand animal cells as hosts for recombinant DNAmolecules. The develop ment ofother vectors has certainly not stood still over the past four years. This has necessitated a thorough revision ofChapters 3 and 4, which deal with bacteriophage and bacterial plasmid vectors. Numerous techniques for in vitromutagenesis have now been tried and tested allowing me to givecomprehensive coverage ofthisarea in Chapter 2 along with the biochemical techniques used to construct recombinant DNA molecules. Readers with some background knowledge of the approaches to gene cloning will be able to go straight toapart ofthe book in whichthey are specificallyinterested.

Author : Kakoli Bose
Publisher : Springer Nature
ISBN 13 : 9811649871
Total Pages : 315 pages
Book Rating : 4.75/5 ( download)

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Download or read book Textbook on Cloning, Expression and Purification of Recombinant Proteins written by Kakoli Bose and published by Springer Nature. This book was released on 2022-01-25 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.

Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 0309166152
Total Pages : 254 pages
Book Rating : 4.57/5 ( download)

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Download or read book Safety of Genetically Engineered Foods written by National Research Council and published by National Academies Press. This book was released on 2004-07-08 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Author : Masayori Inouye
Publisher : Academic Press
ISBN 13 : 1483273970
Total Pages : 330 pages
Book Rating : 4.76/5 ( download)

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Download or read book Experimental Manipulation of Gene Expression written by Masayori Inouye and published by Academic Press. This book was released on 2014-06-28 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experimental Manipulation of Gene Expression discusses a wide range of host systems in which to clone and express a gene of interest. The aims are for readers to quickly learn the versatility of the systems and obtain an overview of the technology involved in the manipulation of gene expression. Furthermore, it is hoped that the reader will learn enough from the various approaches to be able to develop systems and to arrange for a gene of particular interest to express in a particular system. The book opens with a chapter on the design and construction of a plasmid vector system used to achieve high-level expression of a particular phage regulatory protein normally found in minute amounts in a phage-infected bacterial cell. This is followed by separate chapters on topics such as high-level expression vectors that utilize efficient Escherichia coli lipoprotein promoter as well as various other portions of the lipoprotein gene Ipp; DNA cloning systems for streptomycetes; and the design and application of vectors for high-level, inducible synthesis of the product of a cloned gene in yeast.

Author : C. J. Howe
Publisher : Cambridge University Press
ISBN 13 : 9780521407007
Total Pages : 228 pages
Book Rating : 4.01/5 ( download)

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Download or read book Gene Cloning and Manipulation written by C. J. Howe and published by Cambridge University Press. This book was released on 1995-03-31 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduate, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations."--BOOK JACKET.

Author : Dominic Wong
Publisher : Springer Science & Business Media
ISBN 13 : 0387286799
Total Pages : 227 pages
Book Rating : 4.92/5 ( download)

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Download or read book The ABCs of Gene Cloning written by Dominic Wong and published by Springer Science & Business Media. This book was released on 2007-12-31 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clear and concise, this easy-to-use text offers an introductory course on the language of gene cloning, covering microbial, plant, and animal systems. The essential concepts in biology relevant to the understanding of gene cloning are presented in a well-organized and accessible manner. This updated version of the first edition is an invaluable book for nonscientists as well as scientists with little background knowledge in gene cloning, providing a wealth of information for anyone wishing to gain proficiency in reading and speaking the language of gene cloning.

Author : Terence A. Brown
Publisher : Nelson Thornes
ISBN 13 : 9780748740703
Total Pages : 0 pages
Book Rating : 4.08/5 ( download)

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Download or read book Gene Cloning written by Terence A. Brown and published by Nelson Thornes. This book was released on 1998 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene Cloning provides a basic introduction for students and researchers who have no previous experience of experiments with DNA, and assumes very little prior knowledge on the part of the reader. A three part structure addresses the basic principles of gene cloning, the application of cloning in gene analysis, and the role of gene cloning in research and biotechnology. The book is written in clear, jargon-free language, and is extensively illustrated with two-color line drawings.

Author : Professor of Economics School of Oriental and African Studies Christopher Howe
Publisher :
ISBN 13 : 9780511296536
Total Pages : 278 pages
Book Rating : 4.33/5 ( download)

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Download or read book Gene Cloning and Manipulation written by Professor of Economics School of Oriental and African Studies Christopher Howe and published by . This book was released on 2014-05-14 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.