Peptide Nucleic Acids

Author : Peter E. Nielsen
Publisher : Springer Science & Business Media
ISBN 13 : 1592592902
Total Pages : 270 pages
Book Rating : 4.06/5 ( download)

DOWNLOAD NOW!

Download or read book Peptide Nucleic Acids written by Peter E. Nielsen and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptide nucleic acids (PNAs) have now existed for slightly more than ten years, with the interest in and applications of this pseudopeptide DNA mimic steadily increasing during the entire period. PNAs have rapidly attracted the attention of scientists from a diversity of fields ranging from (bio)organic and biophysical chemistry to prebiotic evolution, and from molecular biology to genetic diagnostics and drug development. Many of the applications take advantage of the unique properties of PNA—an uncharged pseudopeptide—that distinguish this DNA mimic from more traditional DNA analogs. Rather than trying to create a comprehensive collection of all published methods and protocols involving PNA—many of which have not yet been validated— I have decided to concentrate on select protocols that are either very well established by several groups around the world, such as PCR-clamping and in situ hybridization, or on new methods that may have broader future impact. Basic methods for PNA oligomer synthesis and analyses have also been included. I am very grateful to those friends and colleagues who have enthusiastically contributed their work, discussions, and writing, and thereby made this book possible. Peter E. Nielsen v Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix IINTRODUCTION 1 PNA Technology Peter E. Nielsen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II CHEMISTRY 2 Solid Phase Synthesis of PNA Oligomers Frederik Beck. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 3 Synthesis of PNA-Peptide Conjugates Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . . 43 4 Parallel Synthesis of PNA-Peptide Conjugate Libraries Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . .

Author : Peter E. Nielsen
Publisher : Garland Science
ISBN 13 : 9780954523244
Total Pages : 332 pages
Book Rating : 4.45/5 ( download)

DOWNLOAD NOW!

Download or read book Peptide Nucleic Acids written by Peter E. Nielsen and published by Garland Science. This book was released on 2004 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptide Nucleic Acids, Second Edition has been extensively revised, updated, and enlarged to contain many new chapters covering the most recent topics and applications in this fast-moving field. The book contains state-of-the-art protocols and applications on all aspects of peptide nucleic acids. Concepts are clearly explained with each chapter containing concise background information. Written by leading experts in the field, the book is an invaluable and complete reference work on this novel and exciting area.

Author : Michael J Waring
Publisher : Royal Society of Chemistry
ISBN 13 : 1788014286
Total Pages : 432 pages
Book Rating : 4.81/5 ( download)

DOWNLOAD NOW!

Download or read book DNA-targeting Molecules as Therapeutic Agents written by Michael J Waring and published by Royal Society of Chemistry. This book was released on 2018-03-08 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: There have been remarkable advances towards discovering agents that exhibit selectivity and sequence-specificity for DNA, as well as understanding the interactions that underlie its propensity to bind molecules. This progress has important applications in many areas of biotechnology and medicine, notably in cancer treatment as well as in future gene targeting therapies. The editor and contributing authors are leaders in their fields and provide useful perspectives from diverse and interdisciplinary backgrounds on the current status of this broad area. The role played by chemistry is a unifying theme. Early chapters cover methodologies to evaluate DNA-interactive agents and then the book provides examples of DNA-interactive molecules and technologies in development as therapeutic agents. DNA-binding metal complexes, peptide and polyamide–DNA interactions, and gene targeting tools are some of the most compelling topics treated in depth. This book will be a valuable resource for postgraduate students and researchers in chemical biology, biochemistry, structural biology and medicinal fields. It will also be of interest to supramolecular chemists and biophysicists.

Author : Lene Jorgensen
Publisher : John Wiley & Sons
ISBN 13 : 0470688408
Total Pages : 442 pages
Book Rating : 4.03/5 ( download)

DOWNLOAD NOW!

Download or read book Delivery Technologies for Biopharmaceuticals written by Lene Jorgensen and published by John Wiley & Sons. This book was released on 2009-10-23 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in biotechnology have provided scientists with an increasing number of biopharmaceuticals such as novel peptide and protein drugs as well as nucleic acid based drugs for gene therapy. However, successful delivery of these biopharmaceuticals is a major challenge because their molecular properties lead to poor physical and chemical stability in the body and limited membrane permeability. Therefore researchers are developing a range of new delivery technologies and materials to enable these new drugs to be delivered intact to their target sites. Delivery Technologies for Biopharmaceuticals describes strategies to overcome the main barriers for successful delivery of therapeutic peptides, proteins, and nucleic acid-based drugs or vaccines related to the site of administration and the target site. Many of the approaches described are reported in formulations in current clinical trials as well as in marketed products. Contents include: challenges in delivery of biopharmaceuticals novel formulation approaches for peptide and protein injectables non-viral chemical vectors and viral technology for delivery of nucleic acid based drugs immune response, adjuvants and delivery systems for vaccines several examples of delivery systems for different biopharmaceuticals a critical assessment of delivery technologies for biopharmaceuticals Delivery Technologies for Biopharmaceuticals is an essential single-volume introduction to the technologies used by researchers to ensure efficient delivery of this exciting new class of drugs. It will be of value to researchers and students working in drug delivery, formulation, biopharmaceuticals, medicinal chemistry, and new materials development.

Author : Christopher Janson
Publisher : Springer Science & Business Media
ISBN 13 : 0387329560
Total Pages : 286 pages
Book Rating : 4.67/5 ( download)

DOWNLOAD NOW!

Download or read book Peptide Nucleic Acids, Morpholinos and Related Antisense Biomolecules written by Christopher Janson and published by Springer Science & Business Media. This book was released on 2007-03-06 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is unique to the existing literature in the Peptide Nucleic Acid field, in that it focuses on comparing and contrasting PNA with other available oligonucleotide homologues and considers areas in which these biomolecules could be profitably applied to clinical and diagnostic applications. Part I of the book addresses comparative strengths and weaknesses of various nucleoside homologues. Part II of the book addresses specific translational or clinical applications for PNA and related antisense biomolecules. The editors have succeeded in presenting a balanced yet broad view of the methods available for gene targeting and modification.

Author : Peter E. Nielsen
Publisher : Garland Publishing
ISBN 13 : 9781898486374
Total Pages : 300 pages
Book Rating : 4.79/5 ( download)

DOWNLOAD NOW!

Download or read book Peptide Nucleic Acids written by Peter E. Nielsen and published by Garland Publishing. This book was released on 2003 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Author : Victor A. Bloomfield
Publisher : Sterling Publishing Company
ISBN 13 : 9780935702491
Total Pages : 854 pages
Book Rating : 4.90/5 ( download)

DOWNLOAD NOW!

Download or read book Nucleic Acids written by Victor A. Bloomfield and published by Sterling Publishing Company. This book was released on 2000-04-17 with total page 854 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing a comprehensive account of the structures and physical chemistry properties of nucleic acids, with special emphasis on biological function, this text has been organized to meet the needs of those who have only a basic understanding of physical chemistry and molecular biology.

Author : Eylon Yavin
Publisher :
ISBN 13 : 9783039368860
Total Pages : 154 pages
Book Rating : 4.69/5 ( download)

DOWNLOAD NOW!

Download or read book Peptide Nucleic Acids written by Eylon Yavin and published by . This book was released on 2020-09-10 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book compiles four review articles and four research papers that highlight recent developments in the field of peptide nucleic acid (PNA) chemistry and biomedicine. The review articles encompass a variety of fields related to PNA, emphasizing the versatility of this DNA mimic. Two excellent reviews detail the use of PNA for molecular diagnostics of miRNAs and genetic point mutations (SNPs). Another review provides a comprehensive analysis of the various approaches for gene editing using chemically modified PNA molecules. Lastly, PNA molecules are elegantly described as effective (antisense) antimicrobial agents in the final review. The high binding affinity of PNA to complementary DNA and RNA is highlighted in three research articles. Two articles show how PNA molecules act as splice modulating and RNA masking molecules, separately. In another contribution, the high affinity and achiral characteristics of PNAs are used to developed a stable L-DNA-based catalytic hairpin assembly. Lastly, chemically-modified PNA molecules are shown to be superior probes for SNP detection. Altogether, these studies illustrate how PNA molecules may be useful for a variety of biomedical applications as either therapeutic or diagnostic agents.

Author : Ramon Eritja
Publisher : Walter de Gruyter GmbH & Co KG
ISBN 13 : 311063953X
Total Pages : 380 pages
Book Rating : 4.37/5 ( download)

DOWNLOAD NOW!

Download or read book Nucleic Acids Chemistry written by Ramon Eritja and published by Walter de Gruyter GmbH & Co KG. This book was released on 2021-01-18 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book compiles recent research on the modification of nucleic acids. It covers backbone modifications and conjugation of lipids, peptides and proteins to oligonucleotides and their therapeutic use. Synthesis and application in biomedicine and nanotechnology of aptamers, fluorescent and xeno nucleic acids, DNA repair and artificial DNA are discussed as well.

Author : Bansi D. Malhotra
Publisher : William Andrew
ISBN 13 : 0128135158
Total Pages : 332 pages
Book Rating : 4.50/5 ( download)

DOWNLOAD NOW!

Download or read book Nanomaterials for Biosensors written by Bansi D. Malhotra and published by William Andrew. This book was released on 2017-10-26 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomaterials for Biosensors: Fundamentals and Applications provides a detailed summary of the main nanomaterials used in biosensing and their application. It covers recent developments in nanomaterials for the fabrication of biosensor devices for healthcare diagnostics, food freshness and bioprocessing. The various processes used for synthesis and characterization of nanostructured materials are examined, along with the design and fabrication of bioelectronic devices using nanostructured materials as building blocks. Users will find the fundamentals of the main nanomaterials used in biosensing, helping them visualize a systematic and coherent picture of how nanomaterials are used in biosensors. The book also addresses the role of bio-conjugation of nanomaterials in the construction of nano-biointerfaces for application in biosensors. Such applications, including metal nanoparticles, metal oxide nanoparticles, nanocomposites, carbon nanotubes, conducting polymers and plasmonic nanostructures in biosensing are discussed relative to each nanomaterial concerned. Finally, recent advancements in protein functionalized nanomaterials for cancer diagnostics and bio-imaging are also included. Provides a detailed study on how nanomaterials are used to enhance sensing capabilities in biosensors Explains the properties, characterization methods and preparation techniques of the nanomaterials used in biosensing Arranged in a material-by-material way, making it clear how each nanomaterial should be used